Upregulation of FZD5 in Eosinophilic Chronic Rhinosinusitis with Nasal Polyps by Epigenetic Modification
Jong-Yeup Kim1,2,*, Min-Ji Cha1,2, Young-Seon Park1,2, Jaeku Kang3,4, Jong-Joong Choi1, Seung Min In1, and Dong-Kyu Kim5,6
1Department of Otorhinolaryngology-Head and Neck Surgery, 2Department of Biomedical Informatics, 3Myunggok Medical Research Institute, 4Department of Pharmacology, College of Medicine, Konyang University, Daejeon 35365, Korea, 5Department of Otorhinolaryngology-Head and Neck Surgery, Chuncheon Sacred Heart Hospital, 6Institute of New Frontier Research, Hallym University College of Medicine, Chuncheon 24253, Korea
*Correspondence: k@kyuh.ac.kr
Received October 31, 2018; Revised February 20, 2019; Accepted February 25, 2019.; Published online April 2, 2019.
© Korean Society for Molecular and Cellular Biology. All rights reserved.

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ABSTRACT
Eosinophilic chronic rhinosinusitis with nasal polyps (CRSwNP) is one of the most challenging problems in clinical rhinology. FZD5 is a receptor for Wnt5A, and its complex with Wnt5A contributes to activating inflammation and tissue modification. Nasal polyps and eosinophil/non-eosinophil counts are reported to be directly correlated. This study investigated the expression and distribution of FZD5, and the role of eosinophil infiltration and FZD5 in eosinophilic CRSwNP pathogenesis. The prognostic role of eosinophil levels was evaluated in seven patients with CRSwNP. Fifteen patients with CRS were classified based on the percentage of eosinophils in nasal polyp tissue. Methylated genes were detected using methylCpG-binding domain sequencing, and qRT-PCR and immunohistochemistry were used to detect FZD5 expression in nasal polyp tissue samples. The results showed that mRNA expression of FZD5 was upregulated in nasal polyps. FZD5 expression was significantly higher in nasal polyp samples from patients with eosinophilic CRSwNP than in those from patients with non-eosinophilic CRSwNP, as indicated by immunohistochemistry. Furthermore, inflammatory cytokine levels were higher in eosinophilic CRSwNP-derived epithelial cells than in normal tissues. In conclusion, FZD5 expression in nasal mucosal epithelial cells is correlated with inflammatory cells and might play a role in the pathogenesis of eosinophilic CRSwNP.
Keywords: chronic rhinosinusitis, eosinophils, nasal polyp, nasal polyposis, sinusitis


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31 March 2019 Volume 42,
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