Isolation and Functional Examination of the Long Non-Coding RNA Redrum
Yerim Lee1, Charny Park1,2, Sanghyuk Lee1,2, Daekee Lee1,2,*, and Jaesang Kim1,2,* 
1Department of Life Science, Ewha Womans University, Seoul 03760, Korea, 2Ewha Research Center for Systems Biology, Seoul
03760, Korea
*Correspondence: daekee@ewha.ac.kr (DL); jkim1964@ewha.ac.kr (JK)
Received October 13, 2017; Accepted November 4, 2017.; Published online December 12, 2017.
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ABSTRACT
Here, we report isolation of multiple long non-coding RNAs (lncRNAs) expressed tissue-specifically during murine embryogenesis. One of these, subsequently came to be known as Redrum, is expressed in erythropoietic cells in fetal liver and adult bone marrow. Redrum transcription is also detected during pregnancy in the spleen where extramedullary hematopoiesis takes place. In order to examine the function of Redrum in vivo, we generated a gene-targeted murine model and analyzed its embryonic and adult erythropoiesis. The homozygous mutant embryo showed no apparent deficiency or defect in erythropoiesis. Adult erythropoiesis in bone marrow and in the spleen during pregnancy likewise showed no detectable phenotype as red blood cells matured in normal fashion. The phenotype is in contrast to the reported function of Redrum in vitro, and our observation implies that Redrum plays in vivo an accessory or supplementary role whose loss is compatible with normal erythropoiesis.
Keywords: erythropoiesis, hematopoiesis, long non-coding
RNA, Redrum


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31 December 2017 Volume 40,
Number 12, pp. 889~985

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