Mol. Cells 2018; 41(11): 900  https://doi.org/10.14348/molcells.2018.0312
Transcriptome Profiling and Characterization of Drought-Tolerant Potato Plant (Solanum tuberosum L.)
Ki-Beom Moon1,2, Dong-Joo Ahn1, Ji-Sun Park1, Won Yong Jung1, Hye Sun Cho1, Hye-Ran Kim1, Jae-Heung Jeon1, Youn-il Park2,*, and Hyun-Soon Kim1,*
1Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea,
2Department of Biological Sciences, Chungnam National University, Daejeon, Korea
*Correspondence: yipark@cnu.ac.kr (YIP); hyuns@kribb.re.kr (HSK)
Received July 23, 2018; Revised September 13, 2018; Accepted September 18, 2018.; Published online November 1, 2018.
© Korean Society for Molecular and Cellular Biology. All rights reserved.

This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. To view a copy of this license, visit (http://creativecommons.org/licenses/by-nc-sa/3.0/).
ABSTRACT
Potato (Solanum tuberosum L.) is the third most important food crop, and breeding drought-tolerant varieties is vital research goal. However, detailed molecular mechanisms in response to drought stress in potatoes are not well known. In this study, we developed EMS-mutagenized potatoes that showed significant tolerance to drought stress compared to the wild-type (WT) ‘Desiree’ cultivar. In addition, changes to transcripts as a result of drought stress in WT and droughttolerant (DR) plants were investigated by de novo assembly using the Illumina platform. One-week-old WT and DR plants were treated with -1.8 Mpa polyethylene glycol-8000, and total RNA was prepared from plants harvested at 0, 6, 12, 24, and 48 h for subsequent RNA sequencing. In total, 61,100 transcripts and 5,118 differentially expressed genes (DEGs) displaying up- or down-regulation were identified in pairwise comparisons of WT and DR plants following drought conditions. Transcriptome profiling showed the number of DEGs with up-regulation and down-regulation at 909, 977, 1181, 1225 and 826 between WT and DR plants at 0, 6, 12, 24, and 48 h, respectively. Results of KEGG enrichment showed that the drought tolerance mechanism of the DR plant can mainly be explained by two aspects, the ‘photosyntheticantenna protein’ and ‘protein processing of the endoplasmic reticulum’. We also divided eight expression patterns in four pairwise comparisons of DR plants (DR0 vs DR6, DR12, DR24, DR48) under PEG treatment. Our comprehensive transcriptome data will further enhance our understanding of the mechanisms regulating drought tolerance in tetraploid potato cultivars.
Keywords: differentially expressed genes, drought stress, ethyl-methanesulfonate-induced mutation, potato breeding, RNA-Seq


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31 October 2018 Volume 41,
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