Adverse Effect of Superovulation Treatment on Maturation, Function and Ultrastructural Integrity of Murine Oocytes
Myungook Lee1, Jong Il Ahn2, Ah Ran Lee1, Dong Woo Ko1, Woo Sub Yang1, Gene Lee3, Ji Yeon Ahn1,*,
and Jeong Mook Lim1,2,*
1Department of Agricultural Biotechnology, Seoul National University, Seoul 08826, Korea, 2Research Institutes of Agriculture
and Life Sciences, Seoul National University, Seoul 08826, Korea, 3Laboratory of Molecular Genetics, Dental Research Institute,
School of Dentistry, Seoul National University, Seoul 03080, Korea
*Correspondence: limjm@snu.ac.kr (JML); ahnji@snu.ac.kr (JYA)
Received April 14, 2017; Revised June 28, 2017; Accepted July 5, 2017.; Published online July 31, 2017.
© Korean Society for Molecular and Cellular Biology. All rights reserved.

This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. To view a copy of this license, visit (http://creativecommons.org/licenses/by-nc-sa/3.0/).
ABSTRACT
Regular monitoring on experimental animal management found the fluctuation of ART outcome, which showed a necessity to explore whether superovulation treatment is responsible for such unexpected outcome. This study was subsequently conducted to examine whether superovulation treatment can preserve ultrastructural integrity and developmental competence of oocytes following oocyte activation and embryo culture. A randomized study using mouse model was designed and in vitro development (experiment 1), ultrastructural morphology (experiment 2) and functional integrity of the oocytes (experiment 3) retrieved after PMSG/hCG injection (superovulation group) or not (natural ovulation; control group) were evaluated. In experiment 1, more oocytes were retrieved following superovulation than following natural ovulation, but natural ovulation yielded higher (p < 0.0563) maturation rate than superovulation. The capacity of mature oocytes to form pronucleus and to develop into blastocysts in vitro was similar. In experiment 2, a notable (p < 0.0186) increase in mitochondrial deformity, characterized by the formation of vacuolated mitochondria, was detected in the superovulation group. Multivesicular body formation was also increased, whereas early endosome formation was significantly decreased. No obvious changes in other microorganelles, however, were detected, which included the formation and distribution of mitochondria, cortical granules, microvilli, and smooth and rough endoplasmic reticulum. In experiment 3, significant decreases in mitochondrial activity, ATP production and dextran uptake were detected in the superovulation group. In conclusion, superovulation treatment may change both maturational status and functional and ultrastuctural integrity of oocytes. Superovulation effect on preimplantation development can be discussed.
Keywords: artificial reproductive Technology (ART), development, gonadotrophins, microorganelle function, oocyte maturation


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31 July 2017 Volume 40,
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