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Fig. 5.

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Fig. 5. Depletion of HYOU1 inhibits stemness and the expression of EMT-related proteins in lung cancer cells. (A) CSC spheroid formation of H1299, A549, and NCI-H460 cells transfected with nonspecific siRNA (siCont) or HYOU1 siRNA (siHYOU1). (B) Expression levels of CD133 and HYOU1 in H1299 cells transfected with nonspecific siRNA (siCont) or HYOU1 siRNA (siHYOU1). (C) Bright-field and immunofluorescence images of lung cancer spheroids (NCI-H460 and A549) transfected with nonspecific siRNA (siCont) or HYOU1 siRNA (siHYOU1) and treated with 20 μM gefitinib. The spheroids were stained with 4 μM EthD-1. The images were obtained using the Operetta® High Content Screening System, and the intensity of EthD-1 staining in lung cancer spheroids was analyzed using Harmony software. (D) Bright-field images of NSCLC cells (NCI-H460 and A549) transfected with nonspecific siRNA (siCont) or HYOU1 siRNA (siHYOU1), as assessed by migration assay. (E) Expression levels of α-SMA, collagen I, HYOU1, N-cadherin, and vimentin in in lung cancer spheroids (NCI-H460 and A549) transfected with nonspecific siRNA (siCont) or HYOU1 siRNA (siHYOU1). The data shown are the mean ± SD from three independent experiments; *P < 0.05 compared to the control group.
Mol. Cells 2021;44:50~62 https://doi.org/10.14348/molcells.2020.0212
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