Molecules and Cells

Indexed in /covered by CAS, KoreaScience & DOI/Crossref:eISSN 0219-1032   pISSN 1016-8478

Fig. 3.

Download original image
Fig. 3. Reciprocal crosstalk between NSCLC cells and HUVECs causes increased HYOU1 expression in MCTSs. (A) Bright-field images of 3D spheroids co-cultured with HUVECs and NSCLC cells (NCI-H460 or A549) and spheroids cultured with lung cancer cells alone. The images were obtained using the Operetta® High Content Screening System with a 10× objective. (B) Expression levels of ATF6, HYOU1, IRE1, and pAKT (Ser473) in spheroids co-cultured with HUVECs and NSCLC cells (NCI-H460 or A549) and spheroids cultured with lung cancer cells alone, as assessed by western blot analysis. (C) Multilayer image showing immunofluorescence staining of HYOU1 in NSCLC (NCI-H460 or A549) spheroids co-cultured with HUVECs and Hoechst staining of both cell types. (D) Expression levels of cleaved caspase-3 and HYOU1 in lung cancer cells transfected with nonspecific siRNA (siCont) or HYOU1 siRNA (siHYOU1) and co-cultured with HUVECs with or without 10 μM or 20 μM gefitinib or cisplatin for 72 h, as assessed by western blot analysis.
Mol. Cells 2021;44:50~62 https://doi.org/10.14348/molcells.2020.0212
© Mol. Cells