Molecules and Cells

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Fig. 7.

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Fig. 7. NSM00158 prevented the occurrence of nonunion in vivo. (A) NSM00158 prevented the occurrence of nonunion in vivo. Femoral fractures were generated in 45 mice, which were randomly divided into three groups (n = 15 per group). On the second day after surgery, the mice in the three groups were injected with DMSO, 10 µM NSM00158, or 20 µM NSM00158. Bone fractures and the extent of their healing were determined using X-ray images taken 0 and 30 days after surgery. (B and C) Radiographic scores of the callus formation and bone union. The images of the fractured bones in the mice from the different groups described in Fig. 7A were quantified to determine the scores of callus formation (B) and bone union (C). *P < 0.05, **P < 0.01. (D) NSM00158 treatment increased the protein levels of the Runx2 targets in vivo. Calluses from three mice presenting with nonunion in the DMSO-injected group and three healed mice in both the 10 µM and 20 µM NSM00158 groups were collected, and total protein was isolated. Cell extracts were used to determine the protein levels of CtBP2, OSC, ALPL, COLA1, IBSP, SPP1, and MMP13. GAPDH was used as a loading control.
Mol. Cells 2020;43:517~529
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