Molecules and Cells

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Fig. 6.

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Fig. 6. NSM00158 treatment reversed the effect of the NADH decrease on the binding of the CtBP2-p300-Runx2 complex at the promoters of the Runx2 target genes. HOB1 cells were treated with 1 mM 2-DG, 2.0 µM NSM00158 + 1 mM 2-DG, 200 µm CoCl2, and 2.0 µM NSM00158 + 200 µm CoCl2 for 18 h. The cells were then subjected to ChIP assays using anti-CtBP2 (A), anti-p300 (B), or anti-Runx2 (C) antibodies. The IgG antibody was used as a negative control. qRT-PCR analyses were performed to evaluate their binding at the promoters of the Runx2 target genes, including OSC, ALPL, COLA1, IBSP, SPP1, and MMP13. CUL4A and FOXM1 were used as controls. **P < 0.01, ***P < 0.001.
Mol. Cells 2020;43:517~529
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