Molecules and Cells

Indexed in /covered by CAS, KoreaScience & DOI/Crossref:eISSN 0219-1032   pISSN 1016-8478

Fig. 4.

Download original image
Fig. 4. NSM00158 significantly changed the occupancy of the CtBP2-p300-Runx2 complex at the promoters of the Runx2 target genes. (A) The occupancy of CtBP2 was significantly decreased at the promoters of the Runx2 target genes in the AOB1 cells. AOB1 cells were treated with or without 2.0 ┬ÁM NSM00158 for 18 h and then subjected to ChIP assays using anti-CtBP1, anti-CtBP2, or IgG antibodies. qRT-PCR analyses were performed to evaluate their binding to the promoters of the Runx2 target genes. CUL4A and FOXM1 were used as controls. *P < 0.05, **P < 0.01. (B) The occupancy of p300 at the promoters of the Runx2 target genes was significantly increased in the AOB1 cells. The cells described in Fig. 4A were subjected to ChIP assays using anti-p300 or IgG antibody, followed by qRT-PCR analyses to evaluate their binding to the promoters of the Runx2 target genes. CUL4A and FOXM1 were used as controls. **P < 0.01, ***P < 0.001. (C) The occupancy of Runx2 at the promoters of its target genes was significantly increased in the AOB1 cells. The cells described in Fig. 4A were subjected to ChIP assays using anti-Runx2 or IgG antibody, followed by qRT-PCR analyses to evaluate their binding to the promoters of the Runx2 target genes. CUL4A and FOXM1 were used as controls. ***P < 0.001.
Mol. Cells 2020;43:517~529 https://doi.org/10.14348/molcells.2020.0042
© Mol. Cells