Molecules and Cells

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Fig. 3.

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Fig. 3. NSM00158 significantly increased the mineralization and ALP activity of the cells. (A and B) Effect of NSM00158 on the mineralization in the AOB3 cells. AOB3 cells were grown in osteogenic medium for 14 days, followed by staining with Von Kossa (A) (scale bars = 1 mm) and alizarin red S (B) (scale bars = 1 cm). (C) Effect of NSM00158 on ALP activity in the AOB3 cells. AOB3 cells were grown in DMEM and then lysed in RIPA buffer. Equal volumes of cell lysate and substrate were mixed in a 96-well plate. After incubation at 37°C for 5 min, the reaction was terminated by supplementation with a stop buffer. The ALP activity was determined by measuring the absorbance at 405 nm. *P < 0.05, ***P < 0.001.
Mol. Cells 2020;43:517~529
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