Molecules and Cells

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Fig. 2.

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Fig. 2. NSM00158 significantly induced the expression of bone formation and differentiation genes. (A) The protein levels of bone formation and differentiation genes were significantly decreased in the AOB cells. Proteins were isolated from three HOB cell lines, HOB1, HOB2, and HOB3, and three AOB cell lines, AOB1, AOB2, and AOB3, and the protein levels of CtBP1, CtBP2, OSC, ALPL, COLA1, IBSP, SPP1, MMP13, and CUL4A were measured. GAPDH was used as a loading control. (B-D) NSM00158 significantly upregulated the expression of bone formation and differentiation genes. HOB1 and AOB3 cells were treated with or without 2.0 ┬ÁM NSM00158 for 18 h, and then, measurements of the protein levels (B) and mRNA levels (C and D) of CtBP1, CtBP2, OSC, ALPL, COLA1, IBSP, SPP1, MMP13, and CUL4A were taken. ***P < 0.001.
Mol. Cells 2020;43:517~529 https://doi.org/10.14348/molcells.2020.0042
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