Molecules and Cells

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Fig. 1.

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Fig. 1. Identification of small molecules that disrupted the CtBP2-p300 interaction in the yeast system. (A) CtBP2 interacted with p300 in yeast cells. Different combinations of plasmids were cotransformed into AH109 cells. The interaction between CtBP2 and p300 was determined in SC-TL medium (top panel) or SC-HTL medium (bottom panel). (B) Growth curve of yeast cells coexpressing CtBP2 and p300. Cells used in (A) were grown in SC-HTL medium for 0, 4, 8, 12, 16, 20, or 24 h, followed by OD600 measurement. ***P < 0.001. (C) Schematic representation of the small-molecule screening. AH109 yeast cells expressing pGADT7-CtBP2 and pGBKT7-p300 were grown in SC-HTL medium for 18 h at 30°C. Cells were diluted to OD600= 0.1 using fresh SC-HTL medium and were seeded in equal concentrations in 96-well plates (left). After incubation at 30°C for 24 h (right), the cell density was measured using a Synergy HTX Multi-mode Reader. The red arrow represented an exampled well harboring a candidate compound. Small molecules that inhibited cell growth (OD600< 0.2) were selected as candidates. (D) The chemical structure of NSM00158.
Mol. Cells 2020;43:517~529
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