Molecules and Cells

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Fig. 5.

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Fig. 5. (A) MCF7 cells were transfected with siFosB followed by PL treatment. MTT assay was performed to evaluate death of MCF7 cells (*P < 0.05). (B) PARP cleavage was analyzed by Western blot after siFosB transfection along with PL treatment. (C) Annexin V positive cells were observed under a confocal microscope after siFosB transfection along with PL treatment. Scale bars = 50 μm. (D) Annexin V/PI positive cells were sorted by flow cytometry after siFosB transfection along with PL treatment. In the graph, percentages of cell death were significantly decreased after siFosB transfection along with PL treatment (***P < 0.001).
Mol. Cells 2019;42:884~892 https://doi.org/10.14348/molcells.2019.0088
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