Molecules and Cells

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Fig. 4.

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Fig. 4. (A) MCF7 cells were transfected with FosB plasmid for 16 h followed by treatment with 10 μM PL for 24 h. MTT assay was performed to evaluate death of MCF7 cells. (B) PARP cleavage was analyzed by Western blot after FosB transfection along with PL treatment. (C) Annexin V positive cells were observed under a confocal microscope after FosB transfection along with PL treatment. Scale bars = 50 μm. (D) Annexin V/PI positive cells were sorted by flow cytometry. Negative stained cells for both annexin V and PI are viable cells (lower left). Cells stained positive for annexin V and negative for PI mean early apoptotic stage (lower right). Positively stained cells for both annexin V and PI are at the end stage of apoptosis (upper right). In the graph, percentages of dead or dying cells were significantly increased after FosB transfection along with PL treatment (***P < 0.001).
Mol. Cells 2019;42:884~892 https://doi.org/10.14348/molcells.2019.0088
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