Molecules and Cells

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Fig. 1.

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Fig. 1. (A) MCF7 cells were treated with PL at indicated concentration for 24 h. mRNA and protein levels were estimated by RT-PCR and western blot, respectively. (B) MCF7 cells were immuno-stained with specific antibodies to SETDB1 (green). DAPI was used as a counter staining for nucleus. Scale bars = 50 μm. (C) MCF7 cells were transiently transfected with SETDB1 siRNA for 16 h followed by treatment with 10 μM PL for 24 h. Protein levels of SETDB1 and PARP cleavage were examined by western blot analyses. (D) Cell viability was assessed by MTT assay. Statistical significance is indicated as ***P < 0.001.
Mol. Cells 2019;42:884~892 https://doi.org/10.14348/molcells.2019.0088
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