Molecules and Cells

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Fig. 5.

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Fig. 5. (A) A 630-bp DNA fragment containing the mexT-mexE intergenic region was labeled with 6-fluorescein amidite and then used as a DNA probe. The fluorescence-labeled DNA probe (400 ng) was incubated without or with 200 ng of purified FL MexT. The regions protected by MexT are indicated by red squares (MexT binding site I and II). Nucleotide numbers shown are the relative positions when the transcription start site mexE is designated as +1. (B) Comparison of the MexT-binding sequences. The two putative MexT-binding sequences in the mexEF-oprN regulatory region are shown at the top. The MexT-binding sequences from other promoters of MexT regulons are aligned below. Nucleotides in bold and underlined represent a partially conserved palindromic sequence, suggesting the potential consensus MexT-binding sequence, ATCA(N)7CGAT.
Mol. Cells 2019;42:850~857
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