Molecules and Cells

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Fig. 4.

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Fig. 4. The effect of PKM knockdown on NF-κB (A) and AP-1 (B) reporter activity. Cells were transfected with an NF-κB or AP-1 reporter plasmid and CMV-renilla and then incubated for 48 h. Reporter activity was measured using substrates included in the reporter assay system. Data are shown as mean ± SD of triplicate values from 3 independent experiments and the asterisks indicate a significant difference between shPKM cells and shControl cells (*P < 0.05). (C) Effect of PKM knockdown on the phosphorylation of the p65 protein. Cells were analyzed by Western blotting. All results of Western blotting are shown as mean ± SD for 3 independent experiments and as a bar graph. (D) Effect of PKM knockdown on the expression of NF-κB target genes. Gene expression was analyzed by RT-PCR. (C and D) β-Actin was used to verify equal protein loading and band density was measured using the ImageJ software program. Similar results were obtained from 3 independent experiments and representative blots are shown.
Mol. Cells 2019;42:628~636 https://doi.org/10.14348/molcells.2019.0038
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