Molecules and Cells

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Fig. 3.

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Fig. 3. (A and B) Effect of PKM knockdown on cell cycle. Cells were seeded and incubated for 48 h in 10% serum-supplemented medium and then stained with propidium iodide. Cell cycle was analyzed using Fluorescence Activated Cell Sorting (FACS). Data are shown as mean ± SD of triplicate values from 3 independent experiments and the asterisk indicates a significant difference between shPKM cells and shControl cells (*P < 0.05). (C and D) Effect of PKM knockdown on cell cycle marker proteins. Cells were synchronized by serum starvation for 24 h and incubated for 48 h in 10% serum-supplemented medium and analyzed by Western blotting. Band density was measured using the ImageJ software program and all results of Western blotting are shown as mean ± SD for 3 independent experiments and as a bar graph.
Mol. Cells 2019;42:628~636 https://doi.org/10.14348/molcells.2019.0038
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