Molecules and Cells

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Fig. 2.

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Fig. 2. (A) Expression of PKM1 and PKM2 proteins in knockdown PKM cells (shPKM) or control cells (shControl) was analyzed by Western blotting. Band density was measured using the Image J (NIH) software program. (B) Effect of PKM knock-down on cell growth. Cells were seeded and then incubated for 72 h. Cell growth were measured at an absorbance of 570 nm. (C) Effect of PKM knockdown on anchorage-independent cell growth. Cells were seeded and incubated for 3 weeks. Colonies were counted using a microscope and the Image-Pro Plus software (v.6) program. (D) Effect of PKM knockdown on cell migration. Cells were seeded in transwells and incubated for 48 h. Migrated cells were counted. (B–D) Data are shown as mean ± SD of triplicate values from 3 independent experiments and the asterisks indicate a significant difference between shPKM cells and shControl cells (*P < 0.05, **P < 0.01).
Mol. Cells 2019;42:628~636 https://doi.org/10.14348/molcells.2019.0038
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