Molecules and Cells

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Fig. 1.

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Fig. 1. (A) The expression of the PKM1 protein in normal and TNBC tissues was analyzed by immunohistochemistry. The expression of PKM1 was determined using a microscope and the Image-Pro Plus software (v.6) program. N, normal tissue; T, TNBC tissue. (B) The expressioin of PKM1 and PKM2 proteins in 12 paried TNBC tissues was analyzed by Western blotting. AT, adjacent tissue; T, cancer tissue. β-Actin was used to verify equal protein loading. Band density was measured using the ImageJ software program (National Institutes of Health, USA). Arrows indicate the PKM1 or PKM2 protein. The results of the expression of PKM1 (left panel) and PKM2 (right panel) proteins are shown as a graph. **P < 0.01. (C and D) The expression of PKM1 or PKM2 in normal breast cells and TNBC cells. The expression of PKM1, PKM2, or phosphorylated PKM2 was analyzed by Western blotting (C) or RT-qPCR (D). Similar results were obtained from 3 independent experiments and representative blots are shown.
Mol. Cells 2019;42:628~636
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