Molecules and Cells

Indexed in /covered by CAS, KoreaScience & DOI/Crossref:eISSN 0219-1032   pISSN 1016-8478

Fig. 3.

Download original image
Fig. 3. (A) Representative profiles of normalized tGFP (green) and mCherry (red) expression from IRES bicistronic vector (tGFP-NP-IRES-mCh-NP). (B) Representative profiles of normalized tGFP (green) and mCherry (red) expression from 2A bicistronic vector (tGFP-NP-2A-IRES-mCh-NP). (C and D) Heatmap showing the deviation of mCherry signal from tGFP signal at a single-cell level following IRES- (C) and 2A-mediated (D) expression. Each row represents a cell. Deviation (dev) is color-coded according to the pseudocolor table on the right. (E) Cross-correlation diagram between tGFP and mCh signals. Red line: IRES-mediated expression. Blue line: 2A-mediated expression. Gray shadow: standard error. n = 38 cells from 2 batches for IRES, 52 cells from 3 batches for p2A. (F) Average absolute deviation of IRES- and 2A-mediated expression in HEK293 and Neuro2a cells. (G) Normalized signal ratio of mCh over tGFP of IRES- and 2A-mediated expression in HEK293 and Neuro2a cells. Lower and upper hinges: first and third quartiles. Lower and upper whiskers: smallest and largest value within 1.5 folds of the interquartile range from the hinge. Notch: a 95% confidence interval for median. *P < 0.05; **P < 0.01 by Tukey honestly significant difference test.
Mol. Cells 2019;42:418~425 https://doi.org/10.14348/molcells.2019.2427
© Mol. Cells