Molecules and Cells

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Fig. 2.

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Fig. 2. (A) Representative profiles of normalized tGFP (green) and mCh (red) expression from IRES bicistronic vector (tGFP-NP-IRES-mCh-NP). (B) Representative profiles of normalized tGFP (green) and mCh (red) expression from 2A bicistronic vector (tGFP-NP-2A-IRES-mCh-NP). (C and D) Heatmap showing the deviation of mCh signal from tGFP signal at the single-cell level following IRES- (C) and p2A-mediated (D) expression. Each row represents a cell. Deviation (dev) is color-coded according to the pseudocolor table on the right. (E) Cross-correlation diagram between tGFP and mCh signals. Red line: IRES-mediated expression. Blue line: 2A-mediated expression. Gray shadow: standard error. n = 40 cells from 2 batches for IRES, 60 cells from 3 batches for 2A.
Mol. Cells 2019;42:418~425 https://doi.org/10.14348/molcells.2019.2427
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