Molecules and Cells

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Fig. 5.

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Fig. 5. (A) Schematic diagram of CrebB isoforms. Black bars indicate approximate annealing regions of exon-specific primers. Transcripts including either exon 4 or exon 6 were detected using the ex4 or ex6 primer sets, respectively. Transcripts in orange were detected using the Δex6 primer set and designated collectively as CrebB-Δex6. Exon 2 has not been annotated by FlyBase, but we designed the ex2 primer set based on the previously predicted sequence (). Black asterisks indicate authentic stop codons in CrebB transcripts; orange asterisks mark potential PTCs. Quantitative transcript analyses of CrebB isoforms in Smg6 mutants [Df(3R)BSC494/Smg62a, blue lines] and the heterozygous controls [Df(3R)BSC494/+, gray lines] were performed as described for . Data represent averages ± SEMs (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001 by Student’s t tests. White/black bars, LD cycles. (B) Quantitative transcript analyses of CrebB isoforms in Smg5 mutants [Df(2L)BSC345/Smg5e04233, green bars] and heterozygous control [Df(2L)BSC345/+, gray bars]. Data represent averages ± SEMs (n = 3–6). *p < 0.05, **p < 0.01 by Student’s t tests. (C) Overexpression of CrebB-b isoform rescues disrupted locomotor rhythms in Smg5-depleted flies. Power of rhythmicity in DD locomotor activity was significantly different in flies with Smg5 depletion and CrebB-b overexpression (p < 0.001, by two-way ANOVA). *p < 0.05, ***p < 0.001 by Sidak’s multiple comparison test. Error bars indicate SEMs (n = 30–65).
Mol. Cells 2019;42:301~312
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