Molecules and Cells

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Fig. 4.

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Fig. 4. (A) Representative confocal images of whole-mount brains stained with anti-PDF antibody. All posterior optic tracts from l-LNvs and dorsal projections from s-LNvs appear intact. (B) Quantitative analyses of daily rhythmic expression of clock-relevant transcripts in Smg6 mutants. Adult flies were collected at the indicated time points in LD cycles (lights on at Zeitgeber time zero [ZT0]; lights off at ZT12). Total RNAs were purified from head extracts, and the level of each clock-relevant transcript relative to that of cyc was quantified by real-time PCR. The y axis indicates the percent expression relative to the peak value in the heterozygous control (set as 100%). Data represent averages ± SEMs (n = 3). *p < 0.05 by Student’s t test. White/black bars, LD cycles.
Mol. Cells 2019;42:301~312
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