Molecules and Cells

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Fig. 2.

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Fig. 2. (A) Expression of DsRed by an enhancer-trapping transgene from an Smg5 locus in the brain of an adult fly (Smg5 > DsRed); both l- and s-LNvs were immunostained with an anti-PDF antibody (green). Yellow dashed box in the left panel indicates the region shown at higher magnification on the right. (B) Representative confocal images of whole-mount brains stained with the anti-PDF antibody. All posterior optic tracts from l-LNvs and dorsal projections from s-LNvs in transgenic flies depleted of Smg5 or Smg6 are intact compared with those in control flies (tim > Dcr2 ). (C, D) Representative images (top) and quantitation (bottom) showing that the Pdf > nlsDsRed ::SV40-3′UTR signal is increased in s-LNvs of Upf22-8A flies and decreased in both l- and s-LNvs of ClkJrk flies compared with that in the wild type w1118. ***p < 0.001 by Student’s t tests. Error bars indicate SEMs (n > 16). (E) Conditional depletion of Smg6 from PDF-expressing neurons dampened DD locomotor rhythms. EtOH (ethanol) indicates a vehicle control for the oral administration of 62.5 μM RU486 during the behavioral tests. ***p < 0.001 by one-way ANOVA, Bonferroni’s post hoc test. Error bars indicate SEMs (n = 10–57).
Mol. Cells 2019;42:301~312
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