Molecules and Cells

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Fig. 1.

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Fig. 1. (A) Results of a genetic screen of transgenic lines harboring RNAi against ~40 post-transcriptional regulators that constitute processing bodies. The RNAi transgenes were individually overexpressed along with the RNAi-enhancing Dicer-2 (Dcr2 ) in all tim-expressing clock cells (tim > Dcr2 ) or in PDF-expressing pacemaker neurons (Pdf > Dcr2 ). Circadian periodicity (x axis) and the power of rhythmicity (y axis) in free-running locomotor activity were calculated in individual flies and averaged for each RNAi line (gray dots). Smg5RNAi flies and their controls are indicated by the colored dots. (B) Powers of rhythmicity seen in Smg5 -depleted flies by different GAL4 drivers (shown at top). *p < 0.05, **p < 0.01, ***p < 0.001 by one-way ANOVA with Tukey’s post hoc test. Error bars indicate SEMs (n = 18–57). (C) Depletion of each NMD factor in tim-expressing clock cells significantly decreases behavioral rhythmicity. ***p < 0.001 versus heterozygous tim -GAL4 or RNAi controls by one-way ANOVA. Error bars indicate SEMs (n = 20–47). (D) Overexpression of the dominant-negative UPF1D45B transgene in PDF-expressing neurons was necessary and sufficient to lengthen the periodicity of circadian locomotor rhythms. Pdf-GAL80 represses the UPF1D45B overexpression in PDF-expressing neurons among other tim-expressing cells. ***p < 0.001 by one-way ANOVA, Tukey’s post hoc test. Error bars indicate SEMs (n = 19–57).
Mol. Cells 2019;42:301~312
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