Molecules and Cells

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Fig. 5.

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Fig. 5. (A) Alteration of the Smad gene family in A549 cells overexpressing IGFBP3 were evaluated by qRT-PCR analysis. β-actin was used to normalize for equal loading. (B) Smad4 mRNA and (C) protein expression levels were detected by qRT-PCR and western blotting, respectively, after transduction with IGFBP3. (D) Smad4 mRNA and (E) protein expression levels were detected by qRT-PCR and western blotting, respectively, after transfection of si-BP3. (F, G) The motility of A549 cells transfected with si-Smad4 overexpressing IGFBP3 was evaluated using transwell assays. Error bars represent the mean ± S.E.M. of three independent experiments. (**p < 0.01, ***p < 0.001)
Mol. Cells 2019;42:321~332 https://doi.org/10.14348/molcells.2019.2441
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