Molecules and Cells

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Fig. 2.

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Fig. 2. (A) The absolute numbers of TCRαβ+ T cells from thymus, spleen, IEL or LP were compared amongst SPF, GF and AF mice. (B) Mice were injected with FITC-conjugated anti-CD45 antibody and sacrificed after 5 min. Representative dot plots show the frequency of intravascular cells. (C) CD8αβ+ IELs were analyzed for their frequencies of Vβ 5.1/5.2, Vβ6, Vβ7, Vβ8.1/8.2, Vβ9 and Vβ11 TCRs in the small intestine. (D) IELs harvested from the indicated mice were stained for IL-17A after stimulation with PMA and ionomycin for 4 h. Representative dot plots show the frequencies of IL-17A+ cells in gated CD8αβ+ IELs from the small intestine (left). The graph shows their frequencies (right). (E) IELs were harvested from SPF mice fed either STD or AFD and analyzed IL-17-producing CD8αβ+ IELs as in (D). Graph shows their frequencies. Numbers indicate the frequency of cells in adjacent gates. Each dot represents an individual mouse and horizontal bars indicate mean values. *P < 0.05, **P < 0.01 (one-way ANOVA). NS, non-specific. Error bars indicate SD. IEL, intraepithelial lymphocyte; LP, lamina propria
Mol. Cells 2019;42:313~320
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