Molecules and Cells

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Fig. 4.

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Fig. 4. (A) Schematic of MEF2C mRNA. miR-551a binding site in MEF2C mRNA 3′UTR (2145–2151bp) is shown as a white box. (B and C) Relative expression of MEF2C proteins and mRNA was determined by Western blotting and RT-qPCR in both Hep3B-lenti-miR clones (B) or miR-transfected cells (C). (D) EGFP reporter (pEGFP-MEF2C 3U) containing MEF2C mRNA 3′UTR (2106–2249bp) and mutant reporter lacking miRNA binding site were constructed. (E) Hep3B cells were sequentially transfected with miRNAs and EGFP reporters and relative expressions of EGFP were determined by Western blotting. β-actin was used as loading control. Images are representative from three independent experiments. Data are mean ± SEM or representative from three independent experiments. *P < 0.05
Mol. Cells 2019;42:175~182
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