Molecules and Cells

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Fig. 3.

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Fig. 3. (A and B) The number of spheres and relative gene expressions were analyzed in both Hep3B-lenti-miR clones (A) or miR-transfected cells (B). Cells were seeded in a low attachment 96-well plate and cultured in serum-free medium. One week later, the number of spheres was counted from three different areas. Relative levels of OCT4, NANOG, KLF4, and SOX2 mRNA were analyzed by RT-qPCR. (C) Seventy-two h after 5-FU treatment, the number of spheres and relative gene expression were analyzed between Hep3B-lenti-miR-Ctrl and Hep3B-lenti-miR-551a cells. Images are representative from three independent experiments. Data represent mean ± SEM from three independent experiments. *P < 0.05
Mol. Cells 2019;42:175~182
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