Molecules and Cells

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Fig. 1.

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Fig. 1. (A) After infection with a lentiviral miRNA library, Hep3B cells were exposed to 10 μM of 5-FU until all control cells (Hep3B-lenti-miR-Ctrl) were dead. Surviving clones were isolated and established as 5-FU-resistant Hep3B clones. (B) GFP expression of Hep3B-lenticlones was observed using fluorescence microscopy. The insertion of miRNA gene integrated from lentivirus was analyzed by sequencing of PCR products amplified from genomic DNA (gDNA) using specific primers, and identified as miR-551a in Hep3B-lenti-miR-551a clone. (C) Relative levels of miR-551a between Hep3B-lenti-miR-Ctrl and Hep3B-lenti-miR-551a were quantified by miRNA RT-qPCR. U6 RNA was used for normalization. (D) Hep3B-lenti-miR-Ctrl and Hep3B-lenti-miR-551a cells were exposed to 5-FU (1, 5, 10, 20, and 50 μM) for 72 h, and cell viability was determined by MTT assay. Data represent mean ± SEM from three independent experiments. *P < 0.05
Mol. Cells 2019;42:175~182
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