Molecules and Cells

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Fig. 5.

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Fig. 5. The TNFR1 antagonist TNFR2-SKE (10 mg/kg) or same volume of phosphate buffered saline (PBS control) was administered to DBA/2 mice (n = 5 for each group) by i.p. injections for every 12 days. Mice in the UVB + TNFR2-SKE and UVB alone groups were exposed to UVB irradiation at 180 mJ/cm2 (312 nm) every other day. Ear samples were prepared from the anesthetized mice. (A and B) One of the ear specimens of each animal was paraffin-embedded and cut with a sliding microtome to 5-μm thickness. Tissue sections were subjected to H & E staining and immunohistochemical examination. (A) UVB-induced epidermal hyperproliferation was analyzed using NIS-element software. Arrows indicate the width of the epidermis. Data show mean ± SD (n = 5). Statistical significance between treatment groups is shown by asterisks as follows: *p < 0.05. Representative images are shown at ×100 magnification, scale bar: 20 μm. (B) Immunohistochemical staining for infiltrated neutrophils with monoclonal antibody against the neutrophil marker, NIMP-R14. The number of infiltrated neutrophils was analyzed using the NIS-element software. Data shown are the mean ± SD (n = 5). *p < 0.05. Magnification, ×400; scale bars = 40 μm. (C) Another ear specimen was soaked in 2 N NaBr solution for exfoliating the epidermis and immersed in 0.14% L-DOPA solution for 3 h at room temperature. The number of melanocytes was analyzed using the NIS-element software. Data show mean ± SD (n = 5). **p < 0.01. Magnification, ×1000; scale bars = 80 μm.
Mol. Cells 2019;42:151~160
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