Molecules and Cells

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Fig. 4.

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Fig. 4. (A) In one set of experiments, NIH3T3 cells were pretreated with TNFR2-SKE or DHA as described in Materials and Methods, followed by treatment with 25 ng/ml mouse TNF-α for 1 h. In another set of experiments, cells were treated with 10 μM of DEX, 1 mM of DHA, or various concentrations of TNFR2-SKE for 4 h after UVB irradiation (312 nm wavelength, 15 mJ/cm2). ChIP assay was performed using anti-NF-κB/p65 antibody to confirm NF-κB binding on the promoter region (−657 to −484) of MMP-9. (B and C) Each cell type was treated with 1 mM of DHA or various concentrations of TNFR2-SKE, and then additionally with 25 ng/ml of human or mouse TNF-α for 24 h. The cell lysates were subjected to SDS-PAGE, and then analyzed using western blotting with the antibodies indicated. Band intensity was quantified using ImageJ software.
Mol. Cells 2019;42:151~160
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