Molecules and Cells

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Fig. 2.

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Fig. 2. (A) NIH3T3 cells were incubated for 30 min with various concentrations of TNFR2-SKE, and then treated with 25 ng/ml mouse TNF-α for 10 min in the presence of TNFR2-SKE. (B) Cells were irradiated with UVB (312 nm wavelength, 15 mJ/cm2), followed by treatment with TNFR2-SKE for 4 h. The interaction between TNFR1 and TRAF2 was analyzed using in situ PLA. The graph indicated the intensity of interaction between molecules mentioned above. PLA signals in the cell population (n = 5) were quantified using NIS Elements analysis. The average number of rolling-circle products (RCPs) per cell ± SD is shown. **p < 0.01, ****p < 0.0001. Nuclei were stained with DAPI (blue fluorescence). Magnification, ×600; scale bars = 10 μm.
Mol. Cells 2019;42:151~160
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