Molecules and Cells

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Fig. 3.

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Fig. 3. (A) HUVECs were exposed to L-flow (12 dynes/cm2) for 24 h. After 24 h, HUVECs were incubated with 10 nM wortmannin (Wort) or 2 μM Compound C (C.C) for 1 h before treatment with 1 μM TG for 24 h. Protein level was analyzed by immunoblotting with specific antibodies against cleaved PARP-1, Cleaved Caspase-3, p-AKT, AKT, p-AMPK, AMPK and α-Tubulin. Bar graphs present the densitometric quantification of western blot bands. ANOVA: *p < 0.05; **p < 0.01. (B) HUVECs were exposed to laminar flow (12 dynes/cm2) for overnight, then incubated with wortmannin for 1 h before treatment with 1 μM thapsigargin for 3 h. Protein level was analyzed by immunoblotting with specific antibodies against spliced-XBP1, ATF4, CHOP, p-eIF2α and α-Tubulin. The asterisk (*) indicates a non-specific band detected by the anti-ATF4 antibody. Bar graphs present the densitometric quantification of western blot bands. Results are expressed as means SDs and are representative of three independent experiments. *p < 0.05; **, p < 0.01 (n = 3).
Mol. Cells 2018;41:964~970 https://doi.org/10.14348/molcells.2018.0111
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