Molecules and Cells

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Fig. 1.

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Fig. 1. HUVECs were treated with 1 μM thapsigargin (TG) or 5 μM tuni-camycin (TM) for overnight after exposure to laminar flow (L-flow, 12 dynes/cm2) for 24 h. (A) Protein level was analyzed by immunoblotting with specific antibodies against PARP-1, Cleaved Casp-3 and α-Tubulin. Bar graphs present the densitometric quantification of Western blot bands. Results are expressed as means SDs and are representative of three independent experiments. *p < 0.05; **p < 0.01 compared with control (n = 3). (B) Representative photomicrographs showing TUNEL (apoptotic, green), DAPI (nuclei, blue) signals and their merged images (original magnification ×400). Bar graphs present number of TUNEL positive cells from total endothelial cell counted. Results are expressed as mean ± SDs and are representative of three independent experiments. *p < 0.05; **p < 0.01 compared with control (n = 3).
Mol. Cells 2018;41:964~970 https://doi.org/10.14348/molcells.2018.0111
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