Fig. 5. (A) Experimental outline. (B) Representative FACS plots of LSKs. Donor-derived (CD45.2⁺) LSKs from recipient mice were analyzed by flow cytometry. (C, D) (C) Frequency and (D) number of LSK cells and further separation of HSPCs into HSC, ST-HSC, HPC-2, MPP, and MP cells in BM recipient mice (n = 5–7). (E) Donor-derived peripheral blood proportion at the indicated time points in BM chimeric mice (n = 5–7). (F) Frequency and absolute numbers of B cells, T cells, and myeloid cells (Gr-1⁺ CD11b⁺ cells) in the BM of chimeric mice at 10 weeks after transplantation (n = 5–7). Data is representative of two independent experiments. Data are displayed as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 (Student’s t test).

Mol. Cells 2018;41:753~761 https://doi.org/10.14348/molcells.2018.0191

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