Molecules and Cells

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Fig. 3.

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Fig. 3. A, B) RNF168 was recruited to DNA lesions in ATM-dependent manner. HeLa cells expressing GFP-RNF168 were subjected into a laser micro-irradiation in the presence of PARP1 or ATM inhibitor as indicated. The GFP intensity representing accumulated PARP1 or ATM at the damaged chromatin was measured by Nikon NIS elements program. Data represent mean ± s.e.m., from five cells. (C, D) PAR-binding ability of RNF168 did not affect its translocation to DNA lesions. (E, F) The protein level of PARP1 in damaged chromatin was controlled by RNF168. EGFP-PARP1 and indicated siRNAs (sictrl or siRNF168) were transfected into HeLa cells and then subjected into a laser micro-irradiation. After 3 h, cells were fixed and stained with indicated antibodies. Data represent mean ± s.d., from 30 cells; *P < 0.05. Straight-red lines indicate the laser-induced damaged areas.
Mol. Cells 2018;41:799~807
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