Molecules and Cells

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Fig. 6.

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Fig. 6. (A) Co-IP of BAX and BCL-xL in hTERT-overexpressing HEK 293T/17 cells. Cells were co-transfected with FLAG-BCL-xL and MYC-hTERT, and then lysed in either Triton X-100 or CHAPS buffer. FLAG-BCL-xL was immunoprecipitated with anti-FLAG beads, followed by western blot analysis with the indicated antibodies. No significant differences in BAX/BCL-xL ratios were observed after hTERT overexpression. (B) Co-IP of BAX and BCL-xL with BAD and hTERT overexpression. The cells were lysed with detergent containing Triton X-100. BAD can disrupt BAX/BCL-xL heterodimers, but hTERT did not affect the BAD-dependent BAX/BCL-xL complex ratios. (C) Co-IP experiment of BAK and MCL-1 with hTERT overexpression. (D) Co-IP analysis of BCL-xL and BECN1 with hTERT overexpression. The BECN1/BCL-xL interaction is not altered by hTERT overexpression.
Mol. Cells 2018;41:684~694 https://doi.org/10.14348/molcells.2018.0206
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