Molecules and Cells

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Fig. 4.

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Fig. 4. (A) Effects of hTERT BH3 mutants on telomerase activity. Telomerase-negative U2OS cells were transiently transfected with expression plasmids encoding FLAG-tagged wild-type (WT) of hTERT, MCL-1 binding-defective mutants (L141A, G145E, D146A), or an hTERT BH3 deletion mutant. Telomerase activity was detected by TRAP assay. IC, internal control of the TRAP assay; NC, negative control without telomerase extract. (B) Telomerase activity by anti-apoptotic proteins BCL-xL and MCL-1 using TRAP analysis. HeLa cells were transiently transfected with the indicated constructs. Transient expression of antiapoptotic BCL-2 members did not affect telomerase activity.
Mol. Cells 2018;41:684~694 https://doi.org/10.14348/molcells.2018.0206
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