Molecules and Cells

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Fig. 2.

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Fig. 2. (A) Co-immunoprecipitation (co-IP) analysis of hTERT and BCL-xL or MCL-1. HEK 293T/17 cells were transfected with the indicated plasmids, followed by co-IP analysis of ectopically expressed hTERT and BCL-xL or MCL-1. (B) Reciprocal co-IP analysis of hTERT and BCL-xL or MCL-1 interactions. The asterisk indicates a nonspecific band. (C) Domain mapping of the interaction between hTERT and MCL-1. HEK 293T/17 cells were transfected with the indicated hTERT truncation mutants, followed by co-IP and Western blot analysis with the indicated antibodies. Mapping of the hTERT domain revealed that both TEN and RT domains are important for MCL-1 interaction. The asterisk indicates a nonspecific band. (D) Co-IP of MCL-1 and hTERT BH3 mutants. hTERT BH3 mutants (L141A, G145E, and D146A) were generated by point mutations at key residues of the BH3 core motif. The hTERT BH3 mutants did not fully disrupt interactions with MCL-1.
Mol. Cells 2018;41:684~694 https://doi.org/10.14348/molcells.2018.0206
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